For high precision determination of the Ca and Mg concentration in saltwater
Along with sodium, potassium, chloride and sulphate, calcium (Ca2+) and magnesium (Mg2+) are two key components of natural seawater. Magnesium and, above all, calcium are important growth factors for organisms which form calcareous skeletons, such as hard coral and coralline algae – both elements form the basic substance of the calcareous skeleton. Furthermore, magnesium and calcium are involved in numerous biochemical processes. In saltwater applications, the reduction in the magnesium or calcium concentration caused partly by cellular metabolism and partly by skeleton formation means it is necessary to test the concentration levels on a regular basis and, if required, add more of one or both ions. This makes it possible to guarantee optimum, near-natural living conditions for all organisms and to prevent long-term damage.
In natural seawater, the calcium concentration is 400-410 mg/l (ppm) and the magnesium concentration is 1280-1320 mg/l (ppm). They also have a fixed ratio of 1:3.25 to one another. Due to the chemical and biochemical interdependencies between calcium and magnesium, you should also aim to achieve this concentration ratio in saltwater tanks.
With the high resolution titration test Tropic Marin® Calcium/Magnesium Combitest Professional the calcium and magnesium concentrations can be determined reliably and separately with a resolution of 4 mg/l Ca and 20 mg/l Mg.
• Measurement range: Ca 300 – 498 mg/l (ppm)
• Measurement range: Mg 820 – 1800 mg/l (ppm)
• Accuracy: Ca 4 mg/l
• Accuracy: Mg 20 mg/l
• For determining the Ca and Mg concentration in saltwater aquaria
• Sufficient for approximately 50 applications
How to use:
Note: The calcium concentration is determined first, and then the magnesium concentration is determined using the same sample.
Determining the calcium level:
1. Shake the bottles before use!
2. Rinse out both glass cuvettes with tap water and then several times with aquarium water.
3. Fill each glass cuvette with exactly 5 ml of aquarium water using the dosing syringe. Put one of the two water samples aside as a reference.
4. Place a clean dropper tip onto the 1 ml dosing syringe with red lettering and draw out reagent A up to the 20 marking on the syringe (corresponds to 0.5 ml). Empty the entire amount into the cuvette with the analysis sample.
5. Close the glass cuvette using the stopper and briefly swirl the solution.
6. Then mix reagent B (powder) with the measuring spoon and add 1 level measuring spoon of reagent B to the glass cuvette containing the analysis sample. Swirl the cuvette carefully until the powder has dissolved. The water sample will turn light-blue.
7. Place another clean dropper tip onto the 1 ml dosing syringe with black lettering and draw out 1 ml of reagent C.
8. Now add reagent C from the syringe to the analysis sample drop by drop until the light-blue solution becomes colourless. Swirl the cuvette after each new drop. To assist in detecting the colour change, take the comparison sample (the second cuvette you prepared at point 3) on a white surface and look into both cuvettes from above when they are sitting side by side. You can stop adding drops when the colour change is complete and there is no difference between the analysis sample and the comparison sample.
9. The residual volume of reagent C in the syringe shows the calcium concentration CCa in mg/l (ppm) that can be determined using table I.
Example: If the lower side of the syringe plunger is at 0.46 ml following titration, then the residual volume of reagent C is 0.46 ml. The calcium concentration in the sample is: Ca = 392 mg/l.
10. The residual volume of reagent C in the syringe can be put back into bottle C.
Determining the magnesium level:
11. Place the third clean dropper tip onto the 1 ml dosing syringe with the green plunger and draw out 1 ml of reagent D.
12. Add approx. 0.4 ml of reagent D to the water sample. The water sample will turn light-blue again.
13. Now add the remaining reagent D from the syringe to the water sample drop by drop until the light-blue solution becomes colourless*. Swirl the cuvette after each new drop. To assist in detecting the colour change, take the comparison sample (the second cuvette you prepared at point 3) on a white surface again and look into both cuvettes from above when they are sitting side by side.
14. The residual volume of reagent D in the syringe shows the magnesium concentration CMg in mg/l (ppm) that can be determined using table II.
Example: If the lower side of the syringe plunger is at 0.35 ml following titration, then the residual volume of reagent C is 0.35 ml. The magnesium concentration in the sample is: Mg = 1300 mg/l (ppm).
15. The residual volume of reagent D in the syringe can be put back into bottle D. Rinse out the glass cuvettes, dosing syringes and dropper tips thoroughly with tap water and allow to dry before using them again.
* Note: If the colour change from light-blue to colourless is difficult to detect, we recommend to carry out the measurement under a bright light source which is similar to daylight.
Arrive Alive Guarantee Policy:
We have close to a 99% success rate on our livestock shipping due to our picking and packing techniques.
However, the unfortunate event that an item is dead on arrival you must follow the below procedure to prevent your claim from being declined.
- You must make contact with us via email within 2 hours of receiving your order. You do not need to call us as we are not always able to answer the phone immediately due to the nature of our work with our hands in water majority of the day.
- The email must contain a clear photo of the livestock in the original bag still sealed. The photo must be clear and show the entire bag.
This allows us to review your claim and refund accordingly. Due to the nature of your purchase, should you not make contact within 2 hours, refund claims will not be considered.
Once your claim has been processed a refund will be honoured for the cost of the item EXCLUDING the cost of postage within 72 hours.
Please note that the acclimatisation process and how the livestock settle into your aquarium is your responsibility as this is out of our hands. There are a number of factors out of our control such as
- Existing fish becoming aggressive towards your new additions
- Incorrect water parameters - PH / Ammonia / Nitrite Levels.
- Incorrect acclimatisation process carried out, introduced livestock too soon which will cause too much stress to the livestock.
Therefore we are unable to cover the livestock once they have been delivered and introduced into your aquarium.
Claims will NOT be considered if you miss delivery and your order is taken to the sorting office. Returns are not accepted on livestock.
DX offer a 'Change Your Delivery Date' HOWEVER, YOU MUST NOT CHANGE THE DELIVERY DATE OF YOUR LIVESTOCK ORDER. IF YOU DO YOU WILL VOID YOUR ARRIVE ALIVE GUARANTEE COMPLETELY AND NO CLAIM WILL BE CONSIDERED.
We strongly recommend that you quarantine any new additions before introducing to your main tank. As with any livestock, we cannot be 100% sure of their condition once they leave us, therefore you should always carry out your own quarantine process and monitor the livestock closely before you make the decision to introduce the livestock to your main aquarium.
As of the 1st May 2021, we will be issuing a credit code rather than a monetary refund, should a livestock item not arrive alive. You must still follow the procedure as normal which is stated in our policy, for your claim to be considered.
The credit code will be issued via email to the email address given on your order.
The credit code will be for the value of the livestock only (excluding postage costs)
This only applies if your item is covered by our Arrive Alive Guarantee policy.
The reason we have made this change is because our livestock prices are one of the cheapest across the industry and we want it to stay this way. We do not have any cover or insurance if an animal does not survive the journey to you, so in order for us to maintain our low prices we will now be offering credit notes instead of cash refunds.
If an item is out of stock at the item of packaging your order, we will issue a refund as we were unable to fulfil the item prior to dispatching.
Tank mate compatibility is crucial to a successful and healthy home aquarium. Incompatible species will increase stress in the tank which could result in disease and considerable loss. Use our compatibility charts as a guideline when selecting fish.
Remember, no guarantees can be made about the compatibility or incompatibility of any particular species of fish. Also, particular species within a group of fish vary in temperament and may not correspond with the guidelines below.
Proper acclimation is the key to successful introduction of new aquarium arrivals. The Floating Method is undoubtedly the most widely practiced method among hobbyists. However, a more exact method of acclimation provided by the Drip Method is required for fish, corals or invertebrates sensitive to sudden changes in water chemistry.
The reason why acclimation is necessary is simple: the chemical makeup of the water in which the aquatic life is packaged is different from your aquarium water chemistry (i.e. water temperature, pH, and salinity). Aquatic life such as fish, and especially invertebrates (including corals), are very sensitive to even minor changes in water chemistry. The goal of acclimation is to gradually introduce your new aquatic life to the water chemistry found in your aquarium at a controlled rate to avoid distress.
The acclimation process is complete when equilibrium is reached, where the temporary container housing new arrivals shares the same water chemistry as your aquarium, and your new arrival appears adjusted to these conditions. Since different species have varying levels of tolerance, different methodologies are required to control the rate of change experienced during the acclimation process.
We recommends employing the Floating Method or the Drift Method of acclimation. The Floating Method of acclimation, where aquarium water is added to the floating bag in 1/2 cup increments, is a great choice when acclimating most types of aquarium fish. However, for more sensitive fish, invertebrates, and corals, it is recommended to employ the Drip Method of acclimation. Keep in mind, no matter which acclimation method you choose, be sure to take your time and never rush the process.
The Drip Method is considered more advanced. It is geared toward sensitive aquatic life such as snails, corals, shrimp, sea stars and wrasses. You'll need a drip acclimation kit (sold separately) and must be willing to monitor the entire process. Gather a clean, 3- or 5-gallon bucket designated for aquarium use only. If acclimating both fish and invertebrates, use a separate bucket for each.
- Be patient – never rush the acclimation procedure. The total acclimation time for your new arrival should take no longer than two hours.
- Always follow the acclimation procedure even if your new arrival appears to be dead. Some fish and invertebrates can appear dead when they arrive and will usually revive when the acclimation procedure is followed correctly.
- Never place an airstone into the shipping bag or bucket when acclimating your new arrival. This will increase the pH of the shipping water too quickly and expose your new arrival to lethal ammonia.
- Keep aquarium lights off for at least four hours after the new arrival is introduced into the aquarium.
- Most invertebrates and marine plants are more sensitive than fish to changes in specific gravity. Please acclimate invertebrates to a specific gravity of 1.023-1.025 or severe stress or trauma may result.
- Some live corals produce excess slime when shipped. After the acclimation procedure is followed, hold the coral by the rock or skeletal base and gently shake the coral in the shipping bag before placing into the aquarium. To avoid damage, please remember never to touch the "fleshy" part of a live coral. Many species of coral will not open for several days after introduction into their new home.
In some instances, a new tank mate will be chased and harassed by one or all of your existing tank mates.
Solution 1: A clean plastic spaghetti strainer (found at a local store) can be used to contain a tank bully within the aquarium for several hours until the new arrival adjusts to its surroundings. Just float the perforated plastic basket in the aquarium. Net the tank bully and place in the floating basket for approximately four hours while the new arrival adjusts to your aquarium. Never place the new arrival in this basket; the new specimen must get familiar with your aquarium. By placing the tank bully in a perforated basket, you’ll reduce the stress on the new arrival.
Solution 2: A perforated plastic lighting grid can be purchased at the local hardware store to cut down the width of your aquarium. This grid may be used to section off a small portion of the aquarium to separate territorial or aggressive fish from the newest tank mate.
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